The ERC-funded laboratory of Christof Niehrs studies regulatory DNA and RNA modifications, which play important roles in development & disease. We utilize highly sensitive mass spectrometry (Triple Quadrupole Mass Spec) and stable isotope metabolic labelling to profile epigenetic and epitranscriptomic changes, including 5mC, 5hmC, 5fC, 5caC, m6A, and others.
The successful candidate will study nucleic acid modifying enzymes to unravel how chemical modifications in DNA, RNA and R-loops regulate pluripotency and differentiation of mammalian embryonic stem cells and embryos.
You will use mass spectrometry, genome-edited CRISPR/Cas9 stem cell mutants, as well as various NGS protocols to carry out a multi- dimensional analysis to address:
Which modifications can be identified and how do they change with different cellular states? Where in the genome and in which RNAs do the modifications occur? What are molecular determinants involved in targeting of modifications to specific sites? The project will involve strong interaction with computational biologists. For more information, see https://www.imb.de/research/niehrs/research
For more information about the project, click here.
• Cutting-edge research projects in an international and interdisciplinary environment, with English as the working language
• Support by strong core facilities (www.imb.de/core-facilities) that provide state-of-the-art services in bioinformatics & AI, cytometry, genomics (e.g. single-cell & small RNA Seq), microscopy (e.g. super-resolution & high-throughput confocal imaging), proteomics, protein production, and a full media lab service
• Advanced training in scientific techniques and professional skills
• Active support as your career develops, e.g. with mentorship, successful networking, and acquisition of independent grants
Please see details on the project, information on how to apply, and an online application form at www.imb.de/postdocs. IMB is an equal opportunity employer.
The deadline for applications is 28 January 2021. Interviews will take place online. Interview dates will be announced once available.