Engineer (M / F) in protein purification and proteomics, 12 months, IPBS, Toulouse France

Posted 13 Jan 2022

IPBS

France (Staff Scientist)

https://www.ipbs.fr/


Missions

As part of a collaborative research project, the candidate will be responsible for implementing biochemical techniques for the expression and purification of tagged mycobacterial proteins for a proteomic analysis of their interactomes by mass spectrometry. These approaches will make it possible to study the molecular players involved in lipid transport in mycobacteria.

Activities

  • Choose and implement tagged protein expression protocols
  • Optimize experimental conditions for sample preparation of in vivo cross-linked proteins.
  • Optimize experimental conditions for the purification of protein complexes
  • Conduct proteomic analyzes of affinity purified complexes (AP-MS)
  • Perform bioinformatic analyzes of the proteomic data obtained
  • Ensure a scientific and technological watch
  • Write reports and present results

Skills

Knowledge

In-depth knowledge of protein biochemistry Theoretical knowledge of proteomics required Knowledge of proteomic data analysis tools

Know how

  • Control of the expression of tagged proteins
  • Proficiency of protocol optimization
  • Proficiency of protein complex purification techniques including, for example, tagged proteins

The recruited person must demonstrate

  • Organization, autonomy and management of working time for the realization of the experiments
  • Ability to work in a team
  • Ability to present the results obtained, orally and written reports.

The person recruited should have a Master's degree. An experience in mass spectrometry field of at least 3 years would be a plus.

Work Context

The work will be carried out at the Institute of Pharmacology and Structural Biology (IPBS) (UMR 5089) in the “Proteomics and Mass Spectrometry of Biomolecules” team directed by Dr. Odile Schiltz in collaboration with the “Molecular Mycobacterial Pathogenesis ” team directed by Dr. Christophe Guilhot. The mission will be carried out under the supervision of a researcher from the proteomics team and will require working closely with the biological researchers of the collaborating team. For several years, the team has been developing innovative strategies in proteomics, in order to better understand the interaction dynamics of proteins and their mechanisms of action in order to study protein function. The team is made up of twenty people with complementary expertise in mass spectrometry, biochemistry and bioinformatics, and is equipped with state-of-the-art instrumentation as well as a solid informatic infrastructure.

IPBS is a unit comprising around 250 people spread over 17 research teams and drawing on several technological platforms, located in Toulouse in the south-west of France.

Complementary information

The candidate is asked to send a CV and a cover letter including (if possible) the contact details of one or two referents.

*To apply * Go to Portail Emploi CNRS

How to Apply

https://emploi.cnrs.fr/Offres/CDD/UMR5089-MANDUC-001/Default.aspx